Regulatory

Part:BBa_K363005:Design

Designed by: Noah Young   Group: iGEM10_Johns_Hopkins   (2010-10-25)

A calcium dependent response element binding site for the Crz1 activator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is NOT itself a promoter. It must be inserted upstream of a promoter to function.


Source

This part was derived from calcium dependent response element (CDRE) binding sequence of the FSK2 gene. The consensus sequence for Crz1 binding as described by Yoshimoto 2002 was mutated at non conserved residues to generate this part. This sequence differs by only one base pair from the consensus. The second base, A in the consensus, is a G in this sequence. We estimate it occurs as a Crz1 binding site with ~1/4 the frequency of the consensus.

References

Yoshimoto et al, J Biol Chem. 2002 Aug 23;277(34):31079-88. Epub 2002 Jun 10. Genome-wide analysis of gene expression regulated by the calcineurin/Crz1p signaling pathway in Saccharomyces cerevisiae.